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This invention is a novel approach to performing DNA sequencing-by-synthesis methodology that is anticipated to make DNA sequencing routine, highly accurate and...
This invention is a novel approach to performing DNA sequencing-by-synthesis methodology that is anticipated to make DNA sequencing routine, highly accurate and affordable.
The approach is a silicon-based field effect nanoscale sensor array using a sequence-by-synthesis methodology. PCR is performed locally in each pixel through the use of recently developed methods for heating fluid using a silicon field effect sensor, and then the amplified molecules in the pixel are detected in a label-free electrical manner where the added charge during the synthesis process is detected using the same thin-film SOI transistor.
Cost is significantly reduced since the nucleotides do not need a fluorescent label, there is no need for gel electrophoresis, and the entire machinery of optical signal processing is unnecessary. In addition, since the silicon field effect sensors when used in a nanoscale dimension can be very sensitive and detect the charge from just a few molecules, it is quite feasible that the PCR amplification can be utilized with very few cycles. Reduction of PCR cycling would reduce the usage of costly reagents. The information retrieval can be scaled and the information obtained per unit time is maximized.
This portfolio of microfluidic technologies improves cell culture techniques. Current cell culture techniques have several limitations: they are resource and...
This portfolio of microfluidic technologies improves cell culture techniques. Current cell culture techniques have several limitations: they are resource and process intensive, the culture environment is inconsistent, and manual manipulation is both time consuming and error prone.
These microfluidic inventions enable more consistent cell culture environments; allow use of high throughput devices which provide speed and consistency; conserve cell-cell factors to maintain cell-cell communication; and ultimately reduce operator error.
High Throughput In Vitro Fertilization (IVF) Device compatible with Multi-Head Flow Cytometry Systems
This invention consists of microchannels to incubate oocytes and embryos. Microchannels are aligned to match the multi-head pipettor of a fluid handling robot with the dispenser of flow cytometers. After a certain amount of incubation time, depending on species, sorted sperm are introduced into the microchannels using a flow cytometer. The microchannels direct the movement and location of the sperm toward the oocyte. Once fertilized, embryos are removed from the fluid handling root and are ready for downstream steps. This process is very low shear, can be easily scaled for high throughput and should improve the yield of current processes.
High Throughput Migration Device for Chemotaxis Studies
Most chemotaxis devices cannot be used in high throughput studies. The geometry and surface tension properties of this invention use fluidic resistance to provide convection and a flow barrier, which then enable the development of concentration gradients. Fluidic resistance ensures this gradient is not disturbed, preserving cell-cell factors. The chamber has a cell inlet, a chemoattractant is introduced in the source channel, and a blank medium is introduced in the sink channel.
Microfluidics Well Insert (MWI) for Oocyte/Embryo Culture
To facilitate culture microenvironments for embryos similar to in-vivo, this invention provides a ready-to-use culture system made using soft lithography technology. This invention has two versions: channel-less and channeled. The channel-less version is composed of wells of a size especially tailored for the characteristic size of the particular species of oocyte/embryo being cultured. This allows for a flexible platform that will accommodate a wide variety of animal species. The channeled version has microchannels networked between the wells of the channel-less version, so that cell-cell communication between oocytes/embryos can be maintained during medium change. This invention is particularly useful for culturing zona pellucida-free oocytes and embryos as the well holds the cells together much as the zona pellicuda would with intact oocytes or embryos. This invention is easily scalable and fits into standard culture dishes.
Automatic Top-Off System using a Surface Tension-Based Valve
This liquid handling system automatically fills culture wells to desired levels without operator handling. Liquid is passively added as surface tension drops, maintaining the medium at a constant level without manual manipulation. This has the following benefits:
Open-Close-Open-Close-Open (OCOCO) Microchannel System
Microchannels provide good micro-environments for cell/embryo/oocyte culture and chemical analysis. However microchannels are very inconvenient for loading or accessing samples. This invention uses passive pumping to change mediums or reagents. This invention does not use any connecting channels or tubing, thus eliminating waste. It can be easily expanded for high-throuhput. The figure shows the liquid flowing from small drop to large drop by surface energy forces.
The construction of nanoporous metal-organic frameworks (MOF) by copolymerization of organic molecules with metal ions has received widespread attention in recent...
The construction of nanoporous metal-organic frameworks (MOF) by copolymerization of organic molecules with metal ions has received widespread attention in recent years. These materials are thermally robust and, in many cases, have high porosity. However, recent experiments have shown that some MOFs are not stable when exposed to >4% water, limiting their usefulness.
Coordination bonding overcomes this limitation, requires mild conditions to create frameworks, and brings myriad choices of building blocks. Trifluoromethoxy group, which has been proved on most water repellent polymers and coating materials, reduces the water damage on MOF structures. A water resistant MOF, namely, ZnMOF3 is obtained through both solvothermal synthesis and microwave assisted solvothermal synthesis. It has a comparable vapor adsorbtion capacity with the commonly used MOFs, but does not adsorb moisture at 70 C.
In addition, exposing ZnMOF3 to boiling water vapor for one week does not result in any dramatic X-ray powder pattern change. ZnMOF3 is a potential adsorbent in many industrial applications such as air adsorption.
A radio frequency (RF) coil comprising a plurality of electrically uninterrupted conductive legs, each leg having a first end and a second end, and at least one...
A radio frequency (RF) coil comprising a plurality of electrically uninterrupted conductive legs, each leg having a first end and a second end, and at least one continuous conductor electrically connected to the first ends of the legs. Frequency tuning of the coil is achieved by translating, along the legs, an electrically continuous tuning band that includes a capacitor closed about the axis of the coil in proximity to the conductive legs. Maintaining electrical symmetry of the coil results in tuning ranges of at least 30 percent of the nominal value of the resonant frequency.
A system that incorporates teachings of the present disclosure may include, for example, an apparatus having an outer nozzle operable to discharge an outer stream...
A system that incorporates teachings of the present disclosure may include, for example, an apparatus having an outer nozzle operable to discharge an outer stream of a shell solution, and an inner nozzle operable to discharge an inner stream of a core solution intermixed with a plurality of materials. The outer stream can substantially surrounds the inner stream, thereby forming a combined stream. A plurality of capsules can be formed responsive to a force applied to the combined stream. At least a portion of the plurality of capsules are desirable capsules, each having a core encapsulated by a portion of the shell solution. The core can have at least one of the plurality of materials encapsulated by a portion of the core solution without protruding an outer surface of the portion of the shell solution.
Cancer researchers are focusing their efforts on identifying central pathways that trigger or enhance the invasiveness of tumor cells. One such target is EMMPRIN...
Cancer researchers are focusing their efforts on identifying central pathways that trigger or enhance the invasiveness of tumor cells. One such target is EMMPRIN (Extracellular Matrix Metalloproteinase Inducer). It has been shown that EMMPRIN expression is linked to various cell signaling pathways that lead to an increase in tumor cell vascularization. Researchers are focusing efforts on elucidating the role of EMMPRIN in a number of disorders including cancer, arthritis, tissue repair and numerous inflammation-dependent diseases. DESCRIPTION/DETAILS Basigin is a member of the immunoglobulin superfamily thatis also known as EMMPRIN. Human basigin is expressed as two differentiallyspliced isoforms encoded by a single gene found on chromosome, renamedrespectively as basigin-1 and basigin-2. Attempts to demonstrate specifichemophilic on separate cells, or between soluble forms of recombinant basiginusing surface Plasmon resonance have not been successful, suggesting thatbasigin-2 cannot function as a receptor for itself. In order to identify possible receptors for soluble basiginligand, an affinity purification approach was employed. This approach resulted inthe labeling of several potential interacting proteins, and MALDI MS/MSsequencing of one of these proteins identified a novel isoform of human basigin(basigin-3).
Immunoprecipitation studies using cell fractions revealed thatrBSG interacts with basigin-2 at the cell membrane, and subsequently interactswith the basigin-3 isoform within the cell. Small-interfering RNA (siRNA)knockdown of basigin-2 protein reduced, but did not eliminate, rBSG-mediatedERK activation in HESCs, suggesting that additional cell surface receptors forsoluble basigin may exist. Taken together, these results support the hypothesisthat basigin-2 can function as a receptor for soluble basigin and demonstratethat the hemophilic interactions between basigin proteins are not dependentupon glycosylation of the basigin ligand. It has also been shown that soluble EMMPRIN binds tomembrane-bound EMMPRIN and the bound complex is internalized presenting apotential means to deliver therapeutic compounds to the cell in a highlytargeted manner. A therapeutic scheme isproposed that aims to design EMMPRIN-conjugates designed to deliver cancertherapeutics in a targeted fashion.
Conjugation oftherapeutics to EMMPRIN increases specificity and targets cancers cells cancer (skin, bladder,breast); other inflammatory diseases (arthritis, endometriosis); Upregulationof inflammatory response.
It is possible to design therapeutic agents directed toward human basigin. Possible therapeutic scheme to block tumor progressionor inflammation.
The development of near-field scanning optical microscopy (NSOT) has been driven by the need for an imaging technique that retains the various contrast mechanisms...
The development of near-field scanning optical microscopy (NSOT) has been driven by the need for an imaging technique that retains the various contrast mechanisms afforded by optical microscopy methods while attaining spatial resolution beyond the classical optical diffraction limit.
NSOT, as an extension of near-field scanning optical microscopy (NSOM), amounts to inverting the scattering data collected by the NSOM and reconstructing the sample, instead of taking the raw data as an image of the sample. This invention is a new method to simplify realization of NSOT and reduce mechanical and other noises. This invention also proposes an alternative NSOT modality to improve the experimental feasibility of NSOT.
For ultra-high optical resolution, near-field scanning optical microscopy (NSOM) is currently the photonic instrument of choice. Near-field imaging occurs when a sub-micron optical probe is positioned a very short distance from the sample and light is transmitted through a small aperture at the tip of this probe. The near-field is defined as the region above a surface with dimensions less than a single wavelength of the light incident on the surface. Within the near-field region evanescent light is not diffraction limited and nanometer spatial resolution is possible. This phenomenon enables non-diffraction limited imaging and spectroscopy of a sample that is simply not possible with conventional optical imaging techniques.
In addition, rather than using multiple observation angles or multiple probes, it is possible to collect data in a third dimension using the spectral degree of freedom. The idea of constructing an image in N spatial dimensions by collecting data in (N - 1) spatial dimensions and a spectral dimension has found application in techniques such as optical coherence tomography and synthetic aperture radar.
The Near-field Volume Scanning Optical Tomography (NVSOT) improves upon the existing Near-field Scanning Optical Microscopy (NSOM) modalities by extending the...
The Near-field Volume Scanning Optical Tomography (NVSOT) improves upon the existing Near-field Scanning Optical Microscopy (NSOM) modalities by extending the data acquisition to obtain the tomographic information of the sample without multi-directional measurements or multi-angle illuminations. Three-dimensional imaging is a much desired capability in the field of life sciences and nanotechnology; current techniques require multiple image acquisitions and complicated optical set-up.
Measurement of the volume above the sample with a strongly scattering tip in the NVSOT based system induces higher order interactions which contains the tomographic information required to generate a three-dimensional image of the sample.
Near-field Volume Scanning Optical Tomography (NVSOT) enables collection of tomographic data for the reconstruction of three dimensional images of sub-wavelength scale samples in the near-field of a strongly scattering tip. This technology eliminates the need for multi-directional measurements of the scattered field in the illumination mode or multi-angle illuminations in the collection mode. Scanning the three dimensional volume above the sample amounts to acquiring multiple NSOM images, data independence is ensured by the higher order interactions between the probe tip and the sample.
For optical microscopy and three-dimensional imaging of sub-wavelength scale samples in the fields of:
About our Portfolio
The University of Illinois at Urbana-Champaign is a world-class research institution boasting a respected and accomplished faculty, high national rankings, state-of-the-art facilities, and a history of ground-breaking research. The University's research budget is more than $600 million annually, placing it among the nation's top generators of innovation.
The Office of Technology Management's portfolio of licensable innovations represents the breadth and depth of the University's research enterprise.
Ready-to-Sign Licensing Program
The goal of this program is to facilitate rapid licensing and the transfer of University intellectual property. Standard terms and conditions have already been determined for each of these technologies, as represented in the linked agreements which are are "ready-to-sign." The terms reflected in Ready-to-Sign agreements are only available to qualified parties who complete the required information, sign, and return the agreement without modification to the Office of Technology Management.
Encouraging Economic Development in Illinois
If you will be developing or producing Products that are covered by the RtS License in the state of Illinois, we will waive the 1st year fee. To qualify for this waiver, on Part 1 of the License indicate the Illinois address where you will be developing or making Products covered by the RtS License.